Library name: Mdfr 
New library: Yes 
Plate names: Mdfr3001 – Mdfr3027; 
Plate format: 384-well 
Date plates shipped: (4/28/04) 
Library materials provided by: Schuyler S.  Korban 
Library constructed by: D.O.Gonzalez/L. Vodkin
Library re-arrayed by: N/A 
Organism: Malus × domestica (apple) 
Tissue: Fruit 
Developmental stage: Stage I-Young Fruitlet < 1 cm
Sex: (gender if applicable) 
Host cell: DH10B ampicillin resistant 
Vector: pSPORT 1 
Vector_type: Phagemid (ampicillin resistant) 
RE_3': Not I 
RE_5': Sal I
DESCR: Total RNA was extracted from freeze dried fruit tissue 
Stage I- Young Fruitlet < 1 cm, using the method described by 
Wang and Vodkin (Plant Molecular Biology Reporter 12:132-145, 
1994). Poly(A)+mRNA was isolated from total RNA using the 
PolyATtract mRNA Isolation system III (Promega). The library 
was prepared using the Invitrogen Life Technologies, 
Superscript Plasmid System with gateway technology for cDNA 
Synthesis and cloning.  Complementary DNA was synthesized 
from mRNA using a Poly (dT) sequence with a NotI restriction 
site.  Sal I linker adapters were ligated to the blunt ended 
cDNA fragments followed by restriction with NotI.  The cDNA 
fragments were directionally cloned into the NotI-SalI 
restriction site of the pSPORT 1 vector.  The ligated cDNA 
fragments were transformed into E. coli ElectroMax Dh10B host 
cells.  Transformation efficiency: 10E+06 colony forming units
Average insert size by PCR: 992 bp