Library name: Mdlv
New library: Yes 
Plate names: Mdlv4014 – Mdlv4026; 
Plate format: 384-well 
Date plates shipped: (8/18/04) 
Library materials provided by: Schuyler S.  Korban 
Library constructed by: D.O.Gonzalez/L. Vodkin
Library re-arrayed by: N/A 
Organism: Malus × domestica (apple) 
Tissue: Leaves 
Developmental stage: Stage III
Sex: (gender if applicable) 
Host cell: DH10B ampicillin resistant 
Vector: pBluescript II SK (+) 
Vector_type: Phagemid (ampicillin resistant) 
RE_3': Xho I
RE_5': Eco RI
DESCR: Total RNA was extracted from freeze dried leaf tissue 
Stage III, using the method described by Wang and Vodkin (Plant Molecular Biology Reporter 12:132-145, 1994).  Poly(A)+ mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III (Promega). The library was prepared using the Stratagene pBluescript II XR cDNA library construction kit.  Complementary DNA was synthesized from mRNA using a Poly (dT) sequence with a XhoI restriction site.  EcoRI adapters were ligated to the blunt ended cDNA fragments followed by restriction with XhoI. The cDNA insert is protected from XhoI digestion via methylation during first strand cDNA synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector.  The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. 

Transformation efficiency: 1.2E+07
Average insert Size by PCR: 1205 bp