The Honey Bee EST project is a functional genomics project, led by Prof. Gene Robinson, Department of Entomology, funded by Critical Research Initiative Grant &Burroughs Welcome Fund.

Lib Code:	BB
Lib Name:	Bee Brain Normalized Library, BB16
Organism:	Apis mellifera
Strain:	mixed strains of European bees, predominantly A.m. ligustica
Sex:	female
Organ:	brain
Tissue type:	brain
Develop. stage:	adult worker honey bee
Lab host:	DH10B
Vector:	pT7T3-Pac R.
Site 1:	EcoR1 R.
Site 2:	Not1
Protocol:	The BB16 library was contributed by the Soares laboratory and it was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research6(9): 791-806. RNA was prepared from dissected brains of adult worker bees of various ages and various behavioral groups.

EST data can be searched and retrieved using the following methods:

Gene Ontology Browser
Search the database by entering a keyword to retrieve Gene Ontology recognized terms, and then view Honey Bee EST annotation for each of those terms. The Gene Ontology Consortium documentation is available at GO documentation.

Sequence ID
Enter an EST ID, GENBANK accession ID, or Contig ID to retrieve and download the sequences, view the sequence chromatograph, view the contig structure and the view the sequence annotation.

Gene Association
Search the database by entering a Sequence ID or Human Unigene Number or Gene Symbol to retrieve annotated ESTs for that ID.

Search by BLAST
Cut and paste one or more sequences to BLAST against several EST data sets.

EST Annotations
Search by description: Enter a familiar gene or protein name or descriptive term, to look for closely related sequences in the bee EST collection. This function allows the searching on the description field of all records retrieved from the BLAST comparisons used in annotation.

Contig Viewer
Enter a contig id to view the consensus sequences that match with it.