U of I ESTIMA Butterfly Start
GO Browser Sequence ID Gene Association Search by Blast Annotations Contig Viewer

Welcome to ESTIMA <Help File>

The Butterfly EST project is database for finding and retrieving gene sequences expressed in the Butterfly

Assembly statistics can be found at http://titan.biotec.uiuc.edu/cgi-bin/ESTWebsite/estima_project_statistics?seqSet=butterfly
Annotations can be found at http://titan.biotec.uiuc.edu/butterfly/resources/butterfly_microarray_seq.txt

Lib Code:BF01
Lib Name:BF01: A normalized library from Monarch butterflies was constructed from total RNA extracted from brains of migrant and non-migrant, male and female butterflies.
Organism:Danaus plexippus
Sex:Male and female
Lab host:DH10B
Vector:pBS II SK(+)
Site 1:EcoRI
Site 2:NotI
Protocol:Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research6(9): 791-806, Genome Research6(9): 791-806

EST data can be searched and retrieved using the following methods:

Gene Ontology Browser
Search the database by entering a keyword to retrieve Gene Ontology recognized terms, and then view Butterfly EST annotation for each of those terms. The Gene Ontology Consortium documentation is available at GO documentation.

Sequence ID
Enter an EST ID, GENBANK accession ID, or Contig ID to retrieve and download the sequences, view the sequence chromatograph, view the contig structure and the view the sequence annotation.

Gene Association
Search the database by entering a Sequence ID or Human Unigene Number or Gene Symbol to retrieve annotated ESTs for that ID.

Search by BLAST
Cut and paste one or more sequences to BLAST against several EST data sets.

EST Annotations
Search by description: Enter a familiar gene or protein name or descriptive term, to look for closely related sequences in the butterfly EST collection. This function allows the searching on the description field of all records retrieved from the BLAST comparisons used in annotation.

Contig Viewer
Enter a contig id to view the consensus sequences that match with it.