U of I ESTIMA Cassava Start
GO Browser Sequence ID Gene Association Search by Blast Annotations Contig Viewer

Welcome to ESTIMA <Help File>

The Cassava EST project is database for finding and retrieving gene sequences expressed in the Euphorbiaceae.

CV01: Normalized library from cassava control. Constructed from 5 samples: mature leaf and petiole, young leaf and apical meristem, root, tuber and tuber peel, young leaf and apical meristem midnight.

CV02: Normalized library from cassava under water stress. Constructed from 4 samples: young leaf and apical meristem, mature leaf and petiole, root, tuber and tuber peel.


EST data can be searched and retrieved using the following methods:

Gene Ontology Browser
Search the database by entering a keyword to retrieve Gene Ontology recognized terms, and then view Cassava EST annotation for each of those terms. The Gene Ontology Consortium documentation is available at GO documentation.

Sequence ID
Enter an EST ID, GENBANK accession ID, or Contig ID to retrieve and download the sequences, view the sequence chromatograph, view the contig structure and the view the sequence annotation.

Gene Association
Search the database by entering a Sequence ID or Human Unigene Number or Gene Symbol to retrieve annotated ESTs for that ID.

Search by BLAST
Cut and paste one or more sequences to BLAST against several EST data sets.

EST Annotations
Search by description: Enter a familiar gene or protein name or descriptive term, to look for closely related sequences in the cassava EST collection. This function allows the searching on the description field of all records retrieved from the BLAST comparisons used in annotation.

Contig Viewer
Enter a contig id to view the consensus sequences that match with it.