The Cattle EST project is a functional genomics project, led by Prof. Harris Lewin, Department of Animal Sciences, sponsored by USDA.

Lib Code:	BP
Lib Name:	Soares normalized bovine placenta
Organism:	Bos taurus
Sex:	female
Organ:	placenta
Lab host:	DH10B
Vector:	pT7T3Pac R.
Site 1:	EcoRI R.
Site 2:	NotI
Protocol:	The cDNA library was contributed by the Soares laboratory and it was constructed and normalized as described by Bonaldo, M.F., Lennon, G. and Soares, M.B. (1996), Genome Research 6(9): 791-806.

Lib Code:	BS
Lib Name:	Subtracted Lewin Cattle Spleen
Organism:	Bos taurus
Strain:	Angus
Sex:	female
Organ:	Spleen Develop.
stage:	Adult
Vector:	pBluescript SK(+/-) R.
Site 1:	EcoRI R.
Site 2:	XhoI
Protocol:	This BS cDNA library was generated by subtraction of the original non-normalized bovine spleen library with 16,800 previously sequenced clones from a bovine Placenta cDNA library. The original library was constructed as described by Band et al (2000), Genome Research10(9): 1359 -1368.

EST data can be searched and retrieved using the following methods:

Gene Ontology Browser
Search the database by entering a keyword to retrieve Gene Ontology recognized terms, and then view Cattle EST annotation for each of those terms. The Gene Ontology Consortium documentation is available at GO documentation.

Sequence ID
Enter an EST ID, GENBANK accession ID, or Contig ID to retrieve and download the sequences, view the sequence chromatograph, view the contig structure and the view the sequence annotation.

Gene Association
Search the database by entering a Sequence ID or Human Unigene Number or Gene Symbol to retrieve annotated ESTs for that ID.

Search by BLAST
Cut and paste one or more sequences to BLAST against several EST data sets.

Contig Viewer
Enter a contig id to view the consensus sequences that match with it.