The Elymus EST project is elymus wheatgrass Pseudoroegnaria spicatais a perennial Triticea grass common to the semi-arid west. It is a diploid, self-incompatible grass that traditionally is slow to establish and intolerant of high grazing pressure. This EST library used tissues from etiolated seedlings, salt and drought stressed roots, and salt and drought stressed shoots and crowns. Bluebunch wheatgrass plants from Eastern Washington and Eastern Nevada were combined, and libraries are tagged by tissues.

EST data can be searched and retrieved using the following methods:

Gene Ontology Browser
Search the database by entering a keyword to retrieve Gene Ontology recognized terms, and then view Elymus EST annotation for each of those terms. The Gene Ontology Consortium documentation is available at GO documentation.

Sequence ID
Enter an EST ID, GENBANK accession ID, or Contig ID to retrieve and download the sequences, view the sequence chromatograph, view the contig structure and the view the sequence annotation.

Gene Association
Search the database by entering a Sequence ID or Human Unigene Number or Gene Symbol to retrieve annotated ESTs for that ID.

Search by BLAST
Cut and paste one or more sequences to BLAST against several EST data sets.

EST Annotations
Search by description: Enter a familiar gene or protein name or descriptive term, to look for closely related sequences in the elymus EST collection. This function allows the searching on the description field of all records retrieved from the BLAST comparisons used in annotation.

Contig Viewer
Enter a contig id to view the consensus sequences that match with it.