The Leymus EST project is leymus ESTs came from rhizome and Spring tiller tissues of basin wildrye Leymus cinereus and beardless wildrye Leymus cinereus F1 hybrids. Rhizome tissues were collected from the horizontal or downward growing points greenhouse soil containers. Purple tillers approximately 10 cm tall were cut at ground level on a freezing cold, early Spring morning near Richmond, UT. Sequencing was done from both 5' and 3' ends of the cDNA clones, and includes a short sequence tag on the 3' end denoting which of the two tissues the sequence originiated.

EST data can be searched and retrieved using the following methods:

Gene Ontology Browser
Search the database by entering a keyword to retrieve Gene Ontology recognized terms, and then view Leymus EST annotation for each of those terms. The Gene Ontology Consortium documentation is available at GO documentation.

Sequence ID
Enter an EST ID, GENBANK accession ID, or Contig ID to retrieve and download the sequences, view the sequence chromatograph, view the contig structure and the view the sequence annotation.

Gene Association
Search the database by entering a Sequence ID or Human Unigene Number or Gene Symbol to retrieve annotated ESTs for that ID.

Search by BLAST
Cut and paste one or more sequences to BLAST against several EST data sets.

EST Annotations
Search by description: Enter a familiar gene or protein name or descriptive term, to look for closely related sequences in the leymus EST collection. This function allows the searching on the description field of all records retrieved from the BLAST comparisons used in annotation.

Contig Viewer
Enter a contig id to view the consensus sequences that match with it.