Leymus ESTs came from rhizome and Spring tiller tissues of basin wildrye Leymus cinereus and beardless wildrye Leymus triticoides F1 hybrids. Rhizome tissues were collected from the horizontal or downward growing points greenhouse soil containers. Purple tillers approximately 10 cm tall were cut at ground level on a freezing cold, early Spring morning near Richmond, UT. Sequencing was done from both 5' and 3' ends of the cDNA clones, and includes a short sequence tag on the 3' end denoting which of the two tissues the sequence originiated.