Cattle functional Genomics group headed by Prof. Harris Lewin. The
High-Throughput Unit has performed 5' end sequencing of a cDNA cattle
spleen and a normalized placenta library with about 17,000 sequences
Honey Bees EST
To accelerate the molecular analysis of behavior in the honey bee (Apis
mellifera), we created an expressed sequence tag (EST) resource for the
bee brain. Over 20,000 cDNA clones were partially sequenced from the
normalized and subtracted libraries at the Keck Center, resulting in
15,311 vector-trimmed, high-quality sequences with an average read length
of 494 bp. and average base-quality of 41. These sequences were assembled
into 8966 putatively unique sequences, which were tested for similarity to
sequences in the public databases with a variety of BLAST searches. Apis
ESTs were tentatively assigned molecular function and biological process
using the Gene Ontology (GO) classification system.
Pig EST resource is constructed by processing more than 100,000 sequences from dbEST and Genbank,
which were compared to sequences in the human Unigene databases with a variety of BLAST searches. These ESTs
were tentatively assigned molecular function and biological process using the Gene Ontology (GO) classification
GenomeProject Management System
GPMS is a web-based interface for management of sequence data for the
large-scale Genome and EST projects through data storage, retrieval, and
analysis. The system integrates a suite of analysis tools that allow users
to access their data from the database, do Blast Searches using in-house
and public databases stored locally, and GenBank submission of their
The COMPASS process is a series of references and association of data.
The result is prediction of locations of a gene or EST on a genome map.
With its up to 95% accuracy, COMPASS can dramatically reduce the time and
cost for genomic mapping.
Keck Center Services
A management software package designed for high throughput oligo
synthesis operation. It has a web interface for request submission, a
management interface for operation, OligoDB, and report generation
High Throughput Data Pickup
High Throughput customers use this link to access the data retrieval sytem.