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BG02: Primary and normalized libraries are constructed using mRNA (processed from total RNA) from bluebunch wheatgrass shoots, roots and seeds. The BG02 library was cloned into pBluescript II SK+. The cloning sites are EcoR1 at the 5’ (EcoR1 adaptor AATTCCGTTGCTGTCG) and NotI at the 3’end. |
Identification of adaptors and tags in 5’-end sequenced clones (using T7 or M13F primer).
Salt/Water Stressed Shoots clones:

Salt/Water Stressed Roots Clones:

Etiolated Seedlings Clones: