Elymus cDNA library

Sample ID	Tag ID	Tag sequence
EL1 (Elymus lanceolatus plant #)	Original	ACGGCCGGG-insert
EW5 (Elymus wawawaiensis plant #5)	GA	ACGGCCGAGGG-insert

 
 
pDONR-LIB (Clontech)
 
Red: 5’ vector sequence
Green: adaptor
Blue: 3’vector sequence
 
EL1 sample:
TCACTATAGGGCGCTAGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTCGACGGTACCGGACATATGCCCGGGAATTCGGCCATTACGGCCGGG

---insertaaaaaaaaaaaaa--

GGCCGCCTCGGCCCTCGAGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCCAGTAGGTAAGTGAACATGGTCATAGCTGTTTCCCTAGGA

EW5 sample:
TCACTATAGGGCGCTAGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTCGACGGTACCGGACATATGCCCGGGAATTCGGCCATTACGGCCGAGGG

---insertaaaaaaaaaaaaa--

GGCCGCCTCGGCCCTCGAGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCCAGTAGGTAAGTGAACATGGTCATAGCTGTTTCCCTAGGA