Library Descriptions


Rhizome

Spring Tillers
  • Leymus
  • BG01: Normalized library from rhizome and Spring tiller tissues of F1 hybrid between basin wildrye and beardless wildrye.


    The BG01 library was cloned into pBluescript II SK+. The cloning sites are EcoR1 at the 5’ (EcoR1 adaptor AATTCCGTTGCTGTCG) and NotI at the 3’end.

    This library has two tags:

    Rhizome clones:



    Spring tiller clones:




    Clone type

    How you read it when sequencing from 5’ end

    How you read it when sequencing from 3’ end

    Rhizome clones

    18(A)TGCGA

    TCGCA18(T)

    Spring tiller clones

    18(A)TCGGA

    TCCGA18(T)