![]() Rhizome |
![]() Spring Tillers |
BG01: Normalized library from rhizome and Spring tiller tissues of F1 hybrid between basin wildrye and beardless wildrye. |
The BG01 library was cloned into pBluescript II SK+. The cloning sites are EcoR1 at the 5’ (EcoR1 adaptor AATTCCGTTGCTGTCG) and NotI at the 3’end.
This library has two tags:
Rhizome clones:
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Spring tiller clones:
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|
Clone type |
How you read it when sequencing from 5’ end |
How you read it when sequencing from 3’ end |
|
Rhizome clones |
18(A)TGCGA |
TCGCA18(T) |
|
Spring tiller clones |
18(A)TCGGA |
TCCGA18(T) |